|
KMID : 0355620180440060259
|
|
Journal of Korean Association of Oral and Maxillofacial Surgeons
2018 Volume.44 No. 6 p.259 ~ p.268
|
|
|
Combined effect of recombinant human bone morphogenetic protein-2 and low level laser irradiation on bisphosphonate-treated osteoblasts
|
|
Jeong Seok-Young
Hong Ji-Un
Song Jae-Min
Kim In-Ryoung
Park Bong-Soo
Kim Chul-Hoon
Shin Sang-Hun
|
|
|
Abstract
|
|
|
Objectives: The purpose of this study was to evaluate the synergic effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) and low-level laser therapy (LLLT) on bisphosphonate-treated osteoblasts.
Materials and Methods: Human fetal osteoblast cells (hFOB 1.19) were cultured with 100 μM alendronate. Low-level Ga-Al-As laser alone or with 100 ng/mL rhBMP-2 was then applied. Cell viability was measured with MTT assay. The expression levels of receptor activator of nuclear factor kappa-B ligand (RANKL), macrophage colony-stimulating factor (M-CSF), and osteoprotegerin (OPG) were analyzed for osteoblastic activity inducing osteoclastic activity. Collagen type and transforming growth factor beta-1 were also evaluated for bone matrix formation.
Results: The results showed that rhBMP-2 and LLLT had a synergic effect on alendronate-treated osteoblasts for enhancing osteoblastic activity and bone matrix formation. Between rhBMP-2 and LLLT, rhBMP-2 exhibited a greater effect, but did not show a significant difference.
Conclusion: rhBMP-2 and LLLT have synergic effects on bisphosphonate-treated osteoblasts through enhancement of osteoblastic activity and bone formation activity.
|
|
|
KEYWORD
|
|
|
Alendronate, Osteoblasts, Bone morphogenetic protein 2, Low-level laser therapy
|
|
|
FullTexts / Linksout information
|
|
  
|
|
|
Listed journal information
|
|
 
|